Semrock offers long-pass fluorescence filter sets

With these long-pass sets, Semrock said more fluorescence signal can be captured for low fluorophore concentration applications and when signal-to-noise ratio is not as critical.

They are also well suited for viewing fluorophores with very wide emission spectra or with a large wavelength shift between the excitation and emission wavelengths.

The filter sets are targeted at imaging the most popular fluorophores including DAPI, GFP, and red fluorescent proteins such as mCherry.

Two DAPI sets are available allowing users to choose between a narrowband excitation filter to minimise photobleaching and phototoxicity to the sample, and a wide exciter for maximum signal in very low concentration samples.

Users of current DAPI, GFP or mCherry Brightline filter sets may quickly upgrade to long-pass sets by dropping in the long-pass emission filters purchased separately.

All three emitters are guaranteed to offer >93 per cent average transmission over the passband and extremely steep edges that are ideal for capturing fluorescence as close to the excitation band as possible for maximum efficiency.

Semrock's hard-coated filter manufacturing process ensures permanent performance with 'no burn-out' even under the most intense illumination conditions.

Semrock filters are available from stock for same-day shipment, while custom-sized filters are available in less than a week.